Clinical study on neutrophil to lymphocyte and platelet ratio with acute kidney injury in elderly patients with sepsis / 中华急诊医学杂志

Objective:The present study aimed to explore the clinical value of neutrophil to lymphocyte and platelet ratio (NLPR) for acute kidney injury (AKI) in elderly patients with sepsis.Methods:This was a retrospective analysis of 360 elderly patients with sepsis or septic shock who were admitted to Intensive Care Unit (ICU) of Zhongnan Hospital of Wuhan University. They were divided into AKI and non-AKI groups based on the KDIGO-AKI criteria. The independent risk factors of AKI were identified via logistic regression analysis, and the calculation of the receiver-operating characteristic (ROC) curves were used to evaluate the diagnostic capability of NLPR for AKI and the short-term outcome of sepsis patients.Results:Total of 195 (54.2%) patients were attributed to the AKI group, while 165 (45.8%) patients were allocated to the non-AKI group. The median of NLPR was significantly higher in the AKI group compared with the non-AKI group ( Z=8.640, P<0.001). Compared with the non-AKI group, the in-hospital death and the length of ICU stay increased (all P<0.05). Patients with AKI required more vasoactive drugs, mechanical ventilation, and renal replacement therapy (all P<0.05). After adjusting the demographic and clinical variables, multivariate logistic regression analysis showed that NLPR was an independent risk factor of AKI ( OR=1.016, 95% CI 1.002-1.030, P=0.027). The ROC curves showed the excellent clinical value of NLPR and which was significantly higher than the neutrophil to lymphocyte ratio (NLR) and serum creatinine. In addition, the present study revealed that the NLPR was also positively correlated with the stage of AKI ( r=0.525, P<0.001). Conclusions:The NLPR is derived from a complete blood cell count, as a new comprehensive inflammatory parameter that is simple and easily available, it is an independent risk factor for AKI in elderly sepsis patients, and should be paid attention in the clinical practice.

G protein?coupled estrogen receptor reduces renal ischemia?reperfusion injury by improving diastolic function of renal interlobular artery / 中华肾脏病杂志

Objective To investigate the effect of G protein?coupled estrogen receptor (GPER) on the diastolic function of renal interlobular artery and reduce renal ischemia?reperfusion injury in rats. Methods Female ovariectomized rats were divided into control group; ischemia?reperfusion injury (IRI) group;GPER?specific agonist (G1) intervention group;GPER?specific blocker+GPER?specific agonist (G15+G1) intervention group. Histopathological examination (HE staining), renal function test and Paller score were used to identify the success of the model and the degree of kidney damage. In vitro microvascular pressure diameter measuring instrument was used to detect the relaxation and contraction activity of renal interlobular artery in each group. Immunofluorescence technique was used to observe the expression of GPER on the renal interlobular artery. Westernblotting was used to detect the expression of GPER protein in renal interlobular artery of rats in each group. The NO content was determined by a nitrate reductase method. Results Compared with IRI group, serum BUN, Scr level and Paller score in G1 intervention group were significantly decreased (all P<0.05). The systolic rate of renal interlobar artery was significantly increased [(40.76 ± 1.57)％ vs (29.78 ± 1.87)％, P<0.05]. The results of immunofluorescence showed that GPER was expressed in renal interlobular artery smooth muscle cells and endothelial cells, and the expression of IRI group was higher than that of the control group. The expression of G15+G1 intervention group was lower than that of G1 intervention group (all P<0.05). Compared with the IRI group, the NO content in the G1 intervention group increased significantly (all P<0.05). Conclusions During renal ischemia ?reperfusion injury, GPER may regulate the systolic and diastolic activity of the renal interlobar artery by increasing the content of NO, so as to alleviate the renal ischemia?reperfusion injury.

Clinical effect ofQiqin decoction combined with lamivudine for the patients with hepatitis B and TCM syndrome of liver stagnation and spleen deficiency and damp heat / 国际中医中药杂志

Objective To discuss the effect ofQiqin decoction combined with lamivudine for the patients with hepatitis B and the TCM syndrome of liver stagnation and spleen deficiency and damp heat. Methods A total of 70 patients with chronic hepatitis B were divided into two groups by random number table method, 35 patients in each group. The patients in the control group were treated with lamivudine, and the patients in the observation group used lamivudine combined withQiqin decoction. The HBV-DNA was detected by fluorescence quantitative PCR, and the levels of serum ALT and AST were detected by full automatic biochemical analyzer. The TCM syndrome score of two groups were compared, and the drug resistance in the treatment process was recorded, and the clinical effect rate was evaluated.Results After treatment, the HBV-DNA negative rate of observation group was 77.1% (27/35), and the control group was 54.3% (19/35). There were statistically significant difference of the negative rate of HBV-DNA between two groups (χ2=4.136, P=0.041). The drug resistance rate of the observation group was 11.4% (4/35), and the control group was 31.4% (11/35). There were statistically significant difference of the drug resistance rate between two groups (χ2=4.297,P=0.043). After treatment, the serum ALT (29.1 ± 13.2 U/Lvs. 121.8 ± 50.2 U/L,t=5.982), the AST (28.5 ± 10.9 U/Lvs. 98.3 ± 46.1 U/L,t=8.263), the TCM syndrome scores (4.8 ± 3.1vs. 11.5 ± 7.9, t=5.982) in the observation group were significantly lower than those in the control group (P<0.01). The total effect rate of observation group was 88.6% (31/35), and the control group was 57.2% (20/35). There was significant difference between two groups (χ2=4.248,P=0.003).Conclusions The Qiqin decoction combined with lamivudine could reduce the levels of HBV-DNA, serum ALT levels and AST levels of patients with chronic hepatitis B, and improve the clinical efficacy.

Establishment of a Zuker diabetic fatty rat model of type II diabetic coronary heart disease / 中国实验动物学报

Objective The purpose of the present study was to develop an animal model of type II diabetic coro-nary heart disease in Zuker diabetic fatty ( ZDF) rats.Methods The ZDF rat model of type II diabetic coronary heart disease was prepared by high-fat diet feeding combined with continuous injection of isoprenaline hydrochloride (ISO) in a dose of 1 mg· mL-1 for 10 consecutive days.Serum creatine kinase (CK), and creatine kinase izozyme (CK-MB), ST segment in electrocardiogram ( ECG) and myocardial pathological changes were detected to evaluate the rat model.Results CK of both the control and model groups was gradually increased with ISO injections, while CK-MB increased first and then decreased.The ST segment in ECG part II had significant changes.The pathological examination found that about half of the myocardial cross section in the model group was necrotic after injections of ISO for 5 days and more than 3/4 of the my-ocardial cross section was necrotic after injection of ISO for 10 days.The results indicated that ISO caused myocardial inju-ry in ZDF rats.Conclusions The variation of CK-MB, CK, ST segments in ECG and myocardial necrosis indicate that the model is successfully established.The use of high-fat diet combined with continuous injection of isoprenaline hydrochlo-ride in a dose of 1 mg· mL is a simple way to develop a Zuker diabetic fatty rat model of type II diabetic coronary heart dis-ease.

Hematologic toxicity of Gynura segetum and effects on vascular endothelium in a rat model of hepatic veno-occlusive disease / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To observe the effects ofGynura segetum in rats with hepatic veno-occlusive disease (HVOD).</p><p><b>METHODS</b>Sixty Sprague-Dawley rats were assigned to a blank control group, one of three Gynura segetum treatment groups (low-dose group, 5.0 g/kg; mid-dose group, 10 g/kg; high-dose group, 20 g/kg), or a pseudo-drug group (10 g/kg of pseudo-ginseng). After 28 days of treatment, effects on white blood cell count, coagulation, secreted factors from vascular endothelium, and histopathology of the spleen were observed and inter-group differences were statistically assessed.</p><p><b>RESULTS</b>After the 4-week administration, all rats in the Gynura segetum treatment groups showed decrease in body weight, increases in numbers of leukocytes, neutrophils, lymphocytes, monocytes and eosinophils.decreases in platelets and platelet hematocrit, and increases in mean platelet volume and platelet distribution.In addition, the Gynura segetum treatments increased the prothrombin time, activated partial thromboplastin time, thrombin time, prothrombin ratio and international normalized ratio, but decreased the PT%, fibrinogen level and platelet aggregation.Serum levels of endothelin and nitric oxide were also elevated by the Gynura segetum treatments.All measured parameters showed significant differences from the control group (P less than 0.01 or less than 0.05).Finally, the splenic follicles were significantly reduced and the spleens showed an absence of germinal centers along with a large number of diffuse lymphocytes and reduced red pulp sinusoids.</p><p><b>CONCLUSION</b>The Gynura segetum treatment has some toxic effects; it can reduce platelet count and platelet hematocrit, inhibit blood clotting time and platelet aggregation, increase the secretion of factors from the vascular endothelium and disrupt spleen histology.</p>

Screening CVB3 VP3 interacting proteins from cDNA library of human heart by yeast two hybridization / 中国人兽共患病学报

To screen interaction proteins of CVB3 VP3 from cDNA library of human heart ,yeast two hybridization was conducted in this study .The bait plasmid pGBKT7-VP3 was constructed ,VP3 fusion protein and its self-activation in AH109 yeast cells was then detected .The positive clones were confirmed by PCR amplification of cDNA inserts ,Alu I digesting ,DNA sequencing ,and Blasting were used to sort positive colonies to eliminate duplicates .Positive clones were confirmed by one-to-one yeast two hybridization ,and them were sequenced and analyzed for homology .Theα-galactosidase assay was performed to detect the interaction strength .Totally ,10 positive proteins interacting with VP3 of CVB3 were obtained by homology analy-sis,namely,EIF4A2,HADHB,GAPDH,ASPG,ACTA1,TNNI3,CKM,LMOD3,ERGIC1,and ALDH2.The strength of interactions between VP3 and 10 candidate proteins were proved byα-galactosidase assay .This study will contribute to explore the CVB3 VP3 function on molecular level and provides some new clues to explain the pathogenic mechanism of myo-carditis and cardiomyopathy .

Diagnosis and treatment of hepatic veno-occlusive disease induced by sedum aizoon in HBsAg positive patients / 中华临床感染病杂志

Objective To review the diagnosis and treatment of hepatic veno-occlusive disease(HVOD)induced by sedum aizoon in HBsAg positive patients. Methods Clinical data of 35 HBsAg positive cases who took sedum aizoon decoction and developed HVOD were collected, the clinical manifestation, imaging examination, histological examination of liver puncture biopsy, and the outcomes of patients were reviewed. Results Hepatomegaly, liver dysfunction, abdominal effusion and map-like density changes in liver CT scan were observed in 35 patients. Liver biopsy wag performed in 17 patients. In histopathological examination, the swelling and point-like necrosis of liver cells, expansion and congestion of sinus, endothelial swelling, wall thickening, incomplete lumen occlusion of small liver vascular were observed. Map-like density changes in liver CT scan were found in all 17 patients who were diagnosed by histological examination. Fifteen patients presented small amount of ascites within 4 weeks of onset, 13 of whom recovered or improved after treated with low-molecular weight heparin and albumin; while among the remaining 20 patients. only half of them were benefited from the same treatments. Conclusion HVOD can be diagnosed by liver CT scan instead of histological examination; treatment of patients in early stage may improve the outcome.

COX-2 or nNOS mediates cardioprotection during the final stage of the late phase of ischemic preconditioning / 生物医学工程学杂志

The aim of the present studies was to investigate the cardioprotection of late IP at 72 h and determine the involvement of iNOS, nNOS and COX-2 in this protection. Conscious rabbits were preconditioned with three cycles of 5-minute coronary occlusion/5-minute reperfusion. The myocardial infarct area in the rabbits preconditioned 72 h earlier was significantly smaller than that in control rabbits. The activity of lactate dehydrogenase (LDH) and the level of 6-Keto-PGF1alpha in the rabbits preconditioned 72 h earlier were lower than those in control rabbits. The left ventricular systolic pressure (LVSP) and maximal velocity of contraction and relaxation (+/- dP/dtmax) were improved in rabbits preconditioned 72 h earlier. The nNOS-selective inhibitors N-propyl-L-arginine and selective cyclooxygenase-2 (COX-2) inhibitor celecoxib completely blocked the protection of late IP at 72 h, whereas the iNOS selective inhibitor S-methybisothiourea had no effect. In conclusion, the cardioprotection observed in the final stage of late IP (72 hours) is mediated by nNOS or COX-2, but not by iNOS.

The research and development of new diagnostic gene chips for SARS coronavirus / 生物医学工程学杂志

The loci of cDNA sequences for valid diagnosis have been identified through the selection of the genome of SARS coronaries. The gene chips for diagnosing such virus have been developed, based on our own-developed technology for manufacturing and application of gene chips. The diagnoses given by such gene chips were consistent well with the reports of clinical laboratories (94.29%) and the sensitivity reached 10(-2)/ml virus molecules. This method is well suited for the clinical use in SARS coronaries diagnosis.

Concentration-response relationship between particulate air pollution and daily mortality in Taiyuan / 北京大学学报(医学版)

Objective:To further study concentration-response relationship between daily mortality and particulate air pollution,to provide scientific basis for making policy.Methods:We applied spline model to daily time-series data for Taiyuan city,China from 2004-2005,using concentration of particulate matter less than 10 ?m in aerodynamic diameter(PM10)as the exposure measure,and controlling confounding factors,such as season,meteorological factors,day-of-the-week,other air pollutions,etc.Results:The spline model showed a non-linear relationship for the relative risks of death for all causes(total deaths)and for specific causes in relation to PM10 concentration,and obtained threshold levels.For total mortality,malignant tumor mortality and cardiovascular-cerebrovascular-respiratory mortality,the threshold levels were 85.00 ?g/m3,89.59 ?g/m3,122.54 ?g/m3,respectively,using the value of differential deduction.Conclusion:Our nonlinear models are appropriate for assessing the effect of particulate air pollution on daily mortality at current ambient levels in Taiyuan,China,in contrast to those of Europe and America.

Establishment of plantlet regeneration system of Rubus idaeus / 中草药

Object\ To establish a plantlet regeneration system of Rubus idaeus L for the purpose to obtain a large number of high quality seedling in a short time Methods\ Stem apex and part of the stem were used as the explant and the optimal culture media and conditions were selected by orthogonal design Results\ An optimum culture medium for the induction of callus, adventitious bud and root was obtained which can be carried out in the laboratory with comparative ease and good repeatability Conclusion\ A basic medium + BA 0 2 mg/L+NAA 1 0～1 5 mg/L was most suitable for the induction of callus; a medium + BA 1 mg/L+NAA 0 1～0 2 mg/L+GA 3 6～8 mg/L+CH 300 mg/L was good for the induction of bud; a medium +BA 1 mg/L+NAA 0 1 mg/L+GA 3 2 mg/L was suitable for propagation of the bud; and the basic medium+IBA 0 1 mg/L+NAA 0 5 mg/L was good for the induction of root

Association of Air Pollution with Preterm Birth in Taiyuan / 环境与健康杂志

Objective To assess the association of air pollution and preterm birth in Taiyuan.Methods The preterm birth data from November 2005 to January 2007 were from Taiyuan birth surveillance system,meteorological data and air pollution data were from Shanxi Meteorological Bureau and Taiyuan Environmental Monitoring Station respectively.The case-crossover analysis and conditional Logistic regression model were used to analyze the association of air pollution and preterm birth in Taiyuan,while the meteorological factors were controlled.Results The stronger effective period of PM10 was 5-day lag and 6-day lag,of SO2 was 7-day lag and 8-days moving average.When PM10,SO2 increased 50 ?g/m3,for PM10,the OR of a 5-day lag and a 6-day lag were 1.169(95%CI:1.058～1.292),1.235(95%CI:1.115～1.367)respectively,for SO2,the OR of a 7-day lag and a 8-days moving average were 1.346(95%CI:1.122～1.616),2.203(95%CI:1.517～3.201)respectively.In sensitivity analysis,the lagged effects of PM10 and the cumulative effects of SO2 were steady.Conclusion The air pollution in Taiyuan may produce an acute effect on preterm birth.

Acute Effect of Air Pollution on Cardiovascular Mortality in Taiyuan / 环境与健康杂志

Objective To analyze quantitatively the acute effect of serious air pollution on cardiovascular mortality in Taiyuan, a typical city where the air was heavy polluted in China. Methods Mortality data in 2004 were from Taiyuan mortality surveillance system, meteorological data and air pollution data were from Taiyuan Meteorological Bureau and Taiyuan Environmental Monitoring Station, respectively. Generalized Additive Model (GAM) extended Poisson regression was used to examine the relationship between air pollution and mortality in Taiyuan, and controlling with time trends, weather and the day of week. Nonparametric conditional bootstrap was used to adjust the concurvity and reduce estimation error. Results The average concentration of PM10, SO2, NO2、CO in 2004 were 173.55, 79.34, 23.03, 2 214.59 ?g/m3 respectively. The median daily count of death for cardiovascular disease in 2004 was 7/daily. Pearson correlation analysis between SO2, CO, PM10 and temperature showed that the association in order were SO2 (r=-0.701 6,P

Exposure-Response Relationship between Particulate Pollution Level and Hospital Outpatient Visits in Taiyuan / 环境与健康杂志

Objective To study the exposure-response relationship between particulate pollution level and hospital outpatient visits. Method From 2001 to 2005,daily outpatient visits data were from a municipal hospital in Taiyuan,meteorological data and air pollution data were from Taiyuan Meteorological Bureau and Taiyuan Environmental Monitoring Station respectively. Poisson Generalized Additive Model(GAM)) extended Poisson regression was used to examine the relationship between particulate pollution level and hospital outpatient visits,controlling with time trends,weather and the day of week. According to the shape of exposure-response relationship curve,piecewise linear regression was used for risk analysis. Results From 2001 to 2005,the average concentration of PM10 was 190.41 ?g/m3,the average number of daily hospital outpatient visits was 18.31 to the respiratory department,and 23.99 to the cardiovascular department. Exposure-response relationship analysis suggested a nonlinear positive correlation between PM10 and daily hospital outpatient visits. A larger increase in daily hospital outpatient visits was observed at lower concentration of PM10 than that at higher concentration. When PM10 increased 10 ?g/m3,respiratory outpatient visits increased 0.260 1%-0.917 8% and respiratory outpatient visits increased 0.208 3%-0.402 7%. The estimated effects of 2 or 3 days moving average of PM10 concentration were greater than that of concurrent day. The stronger effect of PM10 was found on respiratory outpatient visits,which was statistically significant. Conclusion PM10 may produce effect on respiratory outpatient visits.